UCHL5 Antikörper (N-Term)

Produktnummer ABIN357513

Produktdetails anti-UCHL5 Antikörper

Target: UCHL5 (Ubiquitin Carboxyl-terminal Hydrolase L5 (UCHL5))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser UCHL5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Spezifität: This antibody is specific to UCHL5 (N-term).

Aufreinigung: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human UCHL5.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu UCHL5

Target: UCHL5 (Ubiquitin Carboxyl-terminal Hydrolase L5 (UCHL5))

Andere Bezeichnung: UCHL5 (UCHL5 Produkte)

Synonyme: zgc:85615 antikoerper, wu:fj17f09 antikoerper, uch37 antikoerper, cgi-70 antikoerper, DKFZp459M0513 antikoerper, 5830413B11Rik antikoerper, Uch37 antikoerper, CGI-70 antikoerper, INO80R antikoerper, UCH-L5 antikoerper, UCH37 antikoerper, ubiquitin carboxyl-terminal hydrolase L5 antikoerper, ubiquitin C-terminal hydrolase L5 antikoerper, ubiquitin C-terminal hydrolase L5 L homeolog antikoerper, ubiquitin carboxyl-terminal esterase L5 antikoerper, uchl5 antikoerper, UCHL5 antikoerper, uchl5.L antikoerper, Uchl5 antikoerper

Hintergrund: Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54 % identical to that of UCHL1.Synonyms: AD-019, CGI-70, UCH-L5, UCH37, Ubiquitin C-terminal hydrolase UCH37, Ubiquitin carboxyl-terminal hydrolase isozyme L5, Ubiquitin thioesterase L5

Molekulargewicht: 37641 Da

Gen-ID: 51377, 9606

UniProt: Q9Y5K5