PDHX Antikörper (C-Term)

Produktnummer ABIN357414

Dieses Anti-PDHX-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PDHX in WB, IHC (p) und EIA. Geeignet für Human und Maus.

Kurzübersicht für PDHX Antikörper (C-Term) (ABIN357414)

Target: PDHX (Pyruvate Dehydrogenase Complex, Component X (PDHX))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDHX Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-PDHX Antikörper

Bindungsspezifität: C-Term

Spezifität: This antibody detects PDX1 at C-term.

Aufreinigung: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from selected from the C-terminal region of human PDX1.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Details zu PDHX

Target: PDHX (Pyruvate Dehydrogenase Complex, Component X (PDHX))

Andere Bezeichnung: PDHX / PDX1

Hintergrund: PDX1, located in the mitochondrial matrix, is required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex. Eukaryotic pyruvate dehydrogenase complexes are organized about a core consisting of the oligomeric dihydrolipoamide acetyl-transferase, around which are arranged multiple copies of pyruvate dehydrogenase, dihydrolipoamide dehydrogenase and protein X bound by noncovalent bonds. Defects in PDHX are a cause of lacticacidemia. PDX1 belongs to the 2-oxoacid dehydrogenase family and contains 1 lipoyl-binding domain.Synonyms: Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex, E3-binding protein, E3BP, Lipoyl-containing pyruvate dehydrogenase complex component X, Pyruvate dehydrogenase protein X component mitochondrial, proX

Molekulargewicht: 54122 Da

Gen-ID: 8050, 9606

UniProt: O00330