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ISG15 Antikörper (N-Term)

Dieses Anti-ISG15-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ISG15 in WB, IHC (p) und EIA. Geeignet für Human und Maus.
Produktnummer ABIN356679

Kurzübersicht für ISG15 Antikörper (N-Term) (ABIN356679)

Target

Alle ISG15 Antikörper anzeigen
ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Reaktivität

  • 60
  • 6
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Human, Maus

Wirt

  • 56
  • 7
  • 1
Kaninchen

Klonalität

  • 49
  • 15
Polyklonal

Konjugat

  • 44
  • 4
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser ISG15 Antikörper ist unkonjugiert

Applikation

  • 48
  • 35
  • 28
  • 9
  • 7
  • 7
  • 5
  • 2
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • Bindungsspezifität

    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 39-68, N-Term

    Spezifität

    This antibody is specific to ISG15 (N-term).

    Aufreinigung

    Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

    Immunogen

    KLH conjugated synthetic peptide between 39~68 amino acids from the N-terminal region of Human ISG15. Genename: ISG15

    Isotyp

    Ig Fraction
  • Applikationshinweise

    ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    0.25 mg/mL

    Buffer

    PBS containing 0.09 % (W/V) Sodium Azide as preservative.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C/-20 °C

    Informationen zur Lagerung

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Target

    ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

    Andere Bezeichnung

    ISG15 / G1P2

    Hintergrund

    ISG15 is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega , but not IFN-gamma . ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted also by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. The induction of terminal differentiation in human melanoma cells is associated with alterations in ISG15 expression. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog.Synonyms: Interferon-induced 15 kDa protein, Interferon-induced 17 kDa protein, UCRP, Ubiquitin cross-reactive protein

    Gen-ID

    9636, 9606

    UniProt

    P05161
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