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MBD3 Antikörper (C-Term)

This anti-MBD3 antibody (ABIN356576) is a Rabbit Polyclonal antibody detecting MBD3 in WB, IHC (p), EIA. Suitable for Human, Mouse.
Produktnummer ABIN356576

Kurzübersicht für MBD3 Antikörper (C-Term) (ABIN356576)

Target

Alle MBD3 Antikörper anzeigen
MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Reaktivität

  • 66
  • 29
  • 17
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Human, Maus

Wirt

  • 56
  • 8
  • 2
Kaninchen

Klonalität

  • 52
  • 14
Polyklonal

Konjugat

  • 43
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Dieser MBD3 Antikörper ist unkonjugiert

Applikation

  • 42
  • 26
  • 12
  • 8
  • 6
  • 4
  • 3
  • 3
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • Bindungsspezifität

    • 9
    • 7
    • 7
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Spezifität

    This antibody is specific to MBD3 (C-term).

    Aufreinigung

    Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

    Immunogen

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding E279 of human MBD3.

    Isotyp

    Ig Fraction
  • Applikationshinweise

    ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    0.25 mg/mL

    Buffer

    PBS containing 0.09 % (W/V) Sodium Azide as preservative.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C/-20 °C

    Informationen zur Lagerung

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Target

    MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

    Andere Bezeichnung

    MBD3

    Hintergrund

    DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (1). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (2). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (3). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (4).Synonyms: Methyl-CpG-binding domain protein 3

    Gen-ID

    53615, 9606

    UniProt

    O95983

    Pathways

    Chromatin Binding
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