S100A1 Antikörper

Produktnummer ABIN356473

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch S100A1 in IF, IHC (p) und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Human, Ratte, Maus, Rind (Kuh) und Affe.

Kurzübersicht für S100A1 Antikörper (ABIN356473)

Target: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser S100A1 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-S100A1 Antikörper

Spezifität: This antibody stains schwannoma, ependymomas, astrogliomas, almost all benign and malignant melanoma and their metasis. Cellular localization: Cytoplasmic.

Kreuzreaktivität (Details): Species reactivity (expected):Monkey, Rat and Mouse.
Species reactivity (tested):Human, Bovine.

Aufreinigung: Protein A Chromatography

Immunogen: Purified S100 protein from Bovine.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears): 1/50-1/100 usingstreptavidinapprox. biotin system or polymer system. Immunofluorescence. Positive control: Melanoma or schwannoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: 10 mM PO4, 150 mM NaCl, pH 7.4 containing 1 % BSA and 0.05 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: This antibody should not be diluted in Buffer which contains Detergent.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu S100A1

Target: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Andere Bezeichnung: S100A1

Hintergrund: S100 protein is a type of low molecular weight protein found in vertebrates characterized by two calcium binding sites of the helix-loop-helix ("EF-hand type") conformation. There are at least 21 different types of S100 proteins. The name is derived from the fact that the protein is 100 % Soluble in ammonium sulfate at neutral pH . Most S100 proteins are homodimeric, consisting of two identical polypeptides held together by non-covalent bonds. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. Although S100 proteins are structurally similar to calmodulin, they differ in that they are cell-specific, expressed in particular cells at different levels depending on environmental factors. To contrast, calmodulin is a ubiquitous and universal intracellular Ca++ receptor widely expressed in many cells.S-100 is normally present in cells derived from the neural crest (Schwann cells, melanocytes, and glia cells), chondrocyte, adipocytes, myoepithelial cells, macrophages, Langerhans cells, dendritic cells, and keratinocytes. It may be present in some breast epithelial cells.Synonyms: Astrocyte Marker, S-100 protein, S-100 protein alpha chain, S-100 protein alpha subunit, S-100 protein beta chain, S-100 protein beta subunit, S100 Alpha, S100 Beta, S100 Calcium binding protein, S100A, S100B

Gen-ID: 6271

NCBI Accession: NP_006262

UniProt: P23297

Pathways: Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades, S100 Proteine