Fibronectin Antikörper

Produktnummer ABIN356451

Der Kaninchen Polyklonal anti-Fibronectin Antikörper (ABIN356451) detektiert spezifisch Fibronectin in IHC (p) und IHC (fro). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Fibronectin Antikörper (ABIN356451)

Target: Fibronectin

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Fibronectin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Fibronectin Antikörper

Spezifität: This antibody reacts with Human Fibronectin in liver, tonsil, skin and kidney. Cellular localization: Connective tissue matrix and basement membrane.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography on Protein A

Immunogen: Purified human Fibronectin from human plasma.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry: 1/100-1/400 for 30 minute at room temp. using polymer system orstreptavidin-biotin system. Formalin-fixed paraffin-embedded (FFPE) tissue sections andcell smears require antigen retriever, boiling tissue in 10 mM citrate buffer, pH 6.0 for 10-20 minute, followed by cooling at room temp. for 20 minutes. Positive control: Human kidney.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 2.0 mg/mL

Buffer: 10 mM PO4, 150 mM NaCl, pH 7.4, 0.05 % Sodium Azide, 1 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu Fibronectin

Target: Fibronectin

Hintergrund: Fibronectin is a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and extracellular matrix. Fibronectin is involved in cell adhesion and migration process including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. They also occur as dimmer of two 200 kDa subunits. Fibronectin has been implicated in carcinoma development in lung cancer. Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents.Synonyms: CIG, Cold-insoluble globulin, FN1

Gen-ID: 2335

NCBI Accession: NP_002017

UniProt: P02751