CD175s Antikörper (FITC)

Produktnummer ABIN356327

Produktdetails

Target: CD175s

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: FITC

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Produktmerkmale: Synonyms: Sialosyl Tn, Sialosyl-Tn, Sialyl Tn, sTn, NeuAc a GalNAc

Immunogen: Ovine submaxillary mucin (OSM). Remarks: Molecular weight of antigen: Several HM weight glycoproteins. Epitope: NeuAc alphaGalNacOSer/Thr.

Klon: STN218

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears)For IHC dilute concentared antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature. Immunofluorescence: 10-20 μg/mL (1/10-1/20), incubate for 2 hours in the dark at RT or itcan also be incubated overnight at 4 °C. Flow Cytometry: 0.2-1.0 μg/0.1 mL(1/200-1/1,000). Recommended Positive Control: Human gastrointestinal tumor, prostate, ovary ca.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Shelf life: One year from despatch.

Haltbarkeit: 12 months

Antigendetails

Target: CD175s

Hintergrund: Sialosyl Tn antigen also known as CD175s is expressed in the majority of colon, gastric, breast and lung carcinoma. The sialosyl Tn has limited expression to being absent in normal colon, pancreas, stomach, ovary, and prostate. This antigen is detected in several adenocarcinomas. It appears to be useful marker for poorly differentiated colon adenocarcinomas. This antibody stains colon carcinomas and ovarian mucinous carcinomas producing apical cytoplasmic, apical membrane or luminal staining patterns. It is negative with majority of adult tissue sections.Synonyms: NeuAc a GalNAc, Sialosyl Tn, Sialosyl-Tn, Sialyl Tn, sTn