Nestin Antikörper (AA 1464-1614)

Produktnummer ABIN351499

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Nestin in WB, IHC, ICC, IF, IHC (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Nestin Antikörper (AA 1464-1614) (ABIN351499)

Target: Nestin (NES)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nestin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Nestin Antikörper

Bindungsspezifität: AA 1464-1614

Marke: IHC-plus™

Spezifität: This antibody is specific for human nestin. Species cross-reactivity: This antibody recognizes human nestin protein. It does not cross-react with rodent protein.

Keine Kreuzreaktivität: Nagetiere

Aufreinigung: Protein G purified

Immunogen: A fragment of the human nestin protein corresponding to residues 1464-1614.

Type of Immunogen: Protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IF, IHC, IHC-Fr, IHC-P (5 μg/mL), WB (1:1000 - 1:5000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 1:1000-1:5000. Doublet band seen ~220-240kD.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: -20°C.

Details zu Nestin

Target: Nestin (NES)

Andere Bezeichnung: Nestin

Hintergrund: Name/Gene ID: NES

Synonyms: NES, Nestin, Nbla00170

Gen-ID: 10763

UniProt: P48681