Amphiphysin Antikörper (AA 159-170)

Produktnummer ABIN351482

Der Ziege Polyklonal anti-Amphiphysin Antikörper wird verwendet zum Nachweis von Amphiphysin in Proben von Human, Maus, Ratte, Rind (Kuh), Affe, Hund, Pferd, Kaninchen, Hamster und Fledermaus. Er wurde validiert für WB, ELISA, IHC und IHC (p).

Kurzübersicht für Amphiphysin Antikörper (AA 159-170) (ABIN351482)

Target: Amphiphysin (AMPH)

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Affe, Hund, Pferd, Kaninchen, Hamster, Fledermaus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Amphiphysin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Amphiphysin Antikörper

Bindungsspezifität: AA 159-170

Spezifität: Human AMPH / Amphiphysin. This antibody is expected to recognise both reported isoforms (NP_001626.1 and NP_647477.1).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit (100%) Xenopus (92%) Opossum, Chicken, Lizard (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-KRKDESRISKAE from an internal region of human AMPH / Amphiphysin (NP_001626.1, NP_647477.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit (100%), Xenopus (92%), Opossum, Chicken, Lizard (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:16000), WB (0.1 - 0.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Amphiphysin

Target: Amphiphysin (AMPH)

Andere Bezeichnung: AMPH / Amphiphysin

Hintergrund: Name/Gene ID: AMPH

Synonyms: AMPH, AMPH1, Amphiphysin, Amphiphysin I

Gen-ID: 273

NCBI Accession: NP_001626, NP_647477

UniProt: P49418