ADAM12 Antikörper (AA 2-14)

Produktnummer ABIN351478

Dieser Ziege Polyklonal Antikörper detektiert spezifisch ADAM12 in ELISA, WB, IHC und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für ADAM12 Antikörper (AA 2-14) (ABIN351478)

Target: ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ADAM12 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ADAM12 Antikörper

Bindungsspezifität: AA 2-14

Spezifität: Human ADAM12. This antibody is expected to recognise both the longer membrane-bound form of human ADAM12 (NP_003465.3) and the shorter soluble ADAM12 splice isoform (NP_067673.2). This antibody does not cross-react with other ADAMS.

Homologie: Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Monkey (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide AARPLPVSPARALC from the N-terminus of human ADAM12 (NP_003465.3, NP_067673.2). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Monkey (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:64000), WB (0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu ADAM12

Target: ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))

Andere Bezeichnung: ADAM12

Hintergrund: Name/Gene ID: ADAM12
Subfamily: Metallopeptidase M12B
Family: Protease

Synonyms: ADAM12, ADAM 12, MCMPMltna, MCMP, MLTN, Meltrin-alpha, MLTNA

Gen-ID: 8038

NCBI Accession: NP_003465, NP_067673

UniProt: O43184

Pathways: EGFR Signaling Pathway