Sortilin 1 Antikörper (Cytoplasmic Domain)

Produktnummer ABIN350865

Dieses Anti-Sortilin 1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Sortilin 1 in WB und IHC. Geeignet für Human.

Kurzübersicht für Sortilin 1 Antikörper (Cytoplasmic Domain) (ABIN350865)

Target: Sortilin 1 (SORT1)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Sortilin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Sortilin 1 Antikörper

Bindungsspezifität: Cytoplasmic Domain

Verwendungszweck: Rabbit antibody to Sortilin

Spezifität: Specific for Sortilin.

Kreuzreaktivität: Human

Kreuzreaktivität (Details): Other species not yet tested.

Aufreinigung: IgG

Immunogen: A synthetic peptide from the cytoplasmic domain of human Sortilin conjugated to blue carrier protein was used as the antigen.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handhabung: Avoid freeze and thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Haltbarkeit: 12 months

Details zu Sortilin 1

Target: Sortilin 1 (SORT1)

Andere Bezeichnung: Sortilin

Hintergrund: FUNCTION: Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi. SUBUNIT: Interacts with the cytosolic adapter proteins GGA1 and GGA2. Interacts with numerous ligands including the receptor-associated protein LRPAP1/RAP and GM2A LPL PSAP and SLC2A4. Forms a complex with NGFR which binds specifically to the precursor form of NGFB (proNGFB). Interacts with the precursor form of BDNF (proBDNF). SUBCELLULAR LOCATION: Membrane, Single-pass type I membrane protein. Note: Localized to membranes of the endosomes Golgi stack lysosomes and nucleus. Localization to the plasma membrane in adipocytes is enhanced by insulin. Localized to the endoplasmic reticulum and the plasma membrane. Also found in SLC2A4/GLUT4 storage vesicles (GSVs) in adipocytes. TISSUE SPECIFICITY: Expressed at high levels in brain spinal cord heart skeletal muscle thyroid placenta and testis. Expressed at lower levels in lymphoid organs kidney colon and liver.

UniProt: Q99523

Pathways: Neurotrophin Signalübertragung