RHOB Antikörper

Produktnummer ABIN350804

Der Schaf Polyklonal Anti-RHOB-Antikörper wurde für WB und IHC validiert. Er ist geeignet, RHOB in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für RHOB Antikörper (ABIN350804)

Target: RHOB (Ras Homolog Gene Family, Member B (RHOB))

Reaktivität: Human, Maus, Ratte

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser RHOB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-RHOB Antikörper

Verwendungszweck: Sheep antibody to RhoB

Spezifität: Specific for RhOB.

Kreuzreaktivität: Human, Maus, Ratte

Kreuzreaktivität (Details): Other species not yet tested.

Aufreinigung: IgG

Immunogen: A synthetic peptide as a part of human rat mouse Rho-related GTP-binding protein RhoB (ARH6 ARHB H6) conjugated to an immunogenic carrier protein was used as the immunogen.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handhabung: Avoid freeze and thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Haltbarkeit: 12 months

Details zu RHOB

Target: RHOB (Ras Homolog Gene Family, Member B (RHOB))

Andere Bezeichnung: RhoB

Hintergrund: FUNCTION: Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. SUBUNIT: Binds ROCK1 and ROCK2. Also binds PKN1/PRK1. Interacts with ARHGEF3 and AKAP13. Interacts with RTKN. SUBCELLULAR LOCATION: Late endosome membrane, Lipid-anchor. Cell membrane, Lipid-anchor. Nucleus. Note: Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus. INDUCTION: By UV irradiation N-methyl-N-nitrosourea cisplatin cyclohexamide and serum stimulation.

UniProt: P62745