Cofilin Antikörper

Produktnummer ABIN7698698

Der Schaf Polyklonal anti-Cofilin Antikörper wird verwendet zum Nachweis von Cofilin in Proben von Human. Er wurde validiert für WB und IHC.

Kurzübersicht für Cofilin Antikörper (ABIN7698698)

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reaktivität: Human

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser Cofilin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Cofilin Antikörper

Verwendungszweck: Sheep antibody to Cofilin-1

Spezifität: Specific for Cofilin-1.

Kreuzreaktivität: Human, Maus, Ratte

Kreuzreaktivität (Details): Other species not yet tested.

Aufreinigung: IgG

Immunogen: A synthetic peptide from the phospho Y140 & S156 of human Cofilin-1 (Cofilin non-muscle isoform) conjugated to blue carrier protein was used as the antigen. The antigen is homologous in rat mouse and monkey.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user. This antibody may also recognice the unphosphorylated form. Not tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handhabung: Avoid freeze and thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Haltbarkeit: 12 months

Details zu Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Andere Bezeichnung: Cofilin-1

Hintergrund: FUNCTION: Controls reversibly actin polymerization and depolymerization in a pH -sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. SUBCELLULAR LOCATION: Nucleus matrix. Cytoplasm cytoskeleton. Note: Almost completely in nucleus in cells exposed to heat shock or 10 % dimethyl sulfoxide. TISSUE SPECIFICITY: Widely distributed in various tissues.

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events