PCK1 Antikörper

Produktnummer ABIN349649

Der Kaninchen Polyklonal Anti-PCK1-Antikörper wurde für WB validiert. Er ist geeignet, PCK1 in Proben von Mais (Zea mays), Arabidopsis thaliana, Gerste, Nicotiana tabacum, Phaeodactylum tricornutum und Synechocystis zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für PCK1 Antikörper (ABIN349649)

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reaktivität: Mais (Zea mays), Arabidopsis thaliana, Gerste, Nicotiana tabacum, Phaeodactylum tricornutum, Synechocystis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PCK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-PCK1 Antikörper

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 110 / 105 kDa

Aufreinigung: serum

Immunogen: KLH-conjugated synthetic peptide well conserved PEPC1 and sequences from different plant species including Arabidopsis thaliana Q9MAH0, At1g53310 (PEPC 1), Q84VW9, At1g14940 (PEPC 3). The peptide chosen to elicit this antibody is also perfectly conserved in bacterial type of this enzyme NP_177043.2 (PEPC 4)

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: 1 : 1000 with standard ECL (WB).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 200 µL of sterile water.

Buffer: PBS pH 7.4

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Referenzen für anti-PCK1 Antikörper (ABIN349649)

Chang, Jeon, Gu, Pack, Jin: "Conversion of carbon dioxide to oxaloacetate using integrated carbonic anhydrase and phosphoenolpyruvate carboxylase." in: Bioprocess and biosystems engineering, (2013) (PubMed).

Aragón, Pascual, González, Escalona, Carvalho, Amancio: "The physiology of ex vitro pineapple (Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by the environmental conditions: proteomic and transcriptomic profiles." in: Plant cell reports, (2013) (PubMed).

Wostrikoff, Clark, Sato, Clemente, Stern: "Ectopic expression of Rubisco subunits in maize mesophyll cells does not overcome barriers to cell type-specific accumulation." in: Plant physiology, Vol. 160, Issue 1, pp. 419-32, (2012) (PubMed).

Details zu PCK1

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Andere Bezeichnung: Phosphoenolpyruvate Carboxylase

Hintergrund: AGI Code: At1g53310, At1g14940
PEPC (phosphoenolpyruvate carboxylase), EC=4.1.1.31, belongs to an enzyme family of carboxy-lyases that is catalyzing adding fo carbon dioxide to phosphoenolpyruvate (PEP) to form oxaloacetate. Alternative names: PEPCase 1, PEPCase 3, PEPC 1, PEPC 3

Molekulargewicht: expected: 110 kDa, apparent: 105 kDa

NCBI Accession: NP_177043

UniProt: Q84VW9, Q9MAH0

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis