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LacI Antikörper

Dieses Anti--Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von in WB und ELISA. Geeignet für E. coli und Shigella sonnei. Dieses Primary Antibody wurde in 4+ Publikationen zitiert.
Rockland
Produktnummer ABIN349605
Hersteller Produkt- Nr.: 600-401-b04

Kurzübersicht für LacI Antikörper (ABIN349605)

Target

LacI

Reaktivität

  • 1
  • 1
  • 1
E. coli, Shigella sonnei

Wirt

  • 1
Kaninchen

Klonalität

  • 1
Polyklonal

Konjugat

  • 1
Unkonjugiert

Applikation

  • 1
  • 1
  • 1
Western Blotting (WB), ELISA
  • Hersteller Produkt- Nr.

    600-401-b04

    Hersteller

    Rockland

    Verwendungszweck

    Lac I Antibody

    Kreuzreaktivität (Details)

    This antibody reacts with recombinant Lac I protein.

    Produktmerkmale

    Synonyms: rabbit anti-LacI antibody, rabbit anti-Lac-I antibody, Rabbit anti-Lac I antibody, Lactose operon repressor, DNA binding transcriptional repressor antibody, Transcriptional repressor of the lac operon antibody

    Aufreinigung

    This Anti-Lac I product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

    Sterilität

    Sterile filtered

    Immunogen

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant Lac I protein.

    Immunogen Type: Recombinant Protein

    Isotyp

    IgG
  • Applikationshinweise

    Application Note: Rabbit anti-Lac-I antibody has been tested for use in ELISA and western blotting using recombinant Lac I protein.   Specific conditions for reactivity and detection of Lac I should be optimized by the end user.  Expect a band approximately ~38 kDa in size corresponding to Lac I by western blotting in the appropriate cell lysate or extract.

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:90,000 - 1:100,000

    Other: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1.0 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Chang, Thrall, Laureti, Piatt, Pagès, Loparo: "Compartmentalization of the replication fork by single-stranded DNA-binding protein regulates translesion synthesis." in: Nature structural & molecular biology, Vol. 29, Issue 9, pp. 932-941, (2022) (PubMed).

    Kuhn, Pascual-Garcia, Gozalo, Little, Capelson: "Chromatin targeting of nuclear pore proteins induces chromatin decondensation." in: The Journal of cell biology, Vol. 218, Issue 9, pp. 2945-2961, (2020) (PubMed).

    Wyatt, Xiao, Americo, Earl, Moss: "Novel Nonreplicating Vaccinia Virus Vector Enhances Expression of Heterologous Genes and Suppresses Synthesis of Endogenous Viral Proteins." in: mBio, Vol. 8, Issue 3, (2018) (PubMed).

    Agelopoulos, McKay, Mann: "Developmental regulation of chromatin conformation by Hox proteins in Drosophila." in: Cell reports, Vol. 1, Issue 4, pp. 350-9, (2013) (PubMed).

  • Target

    LacI

    Hintergrund

    Background: Anti-Lac I antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The lac repressor protein (Lac I) is an important genetic control protein. It controls the expression of the lactose metabolizing enzymes in Escherichia coli. This regulation involves specific recognition of the operator DNA sequence (Lac O) and consequent inhibition of transcription initiation by RNA polymerase, modulation of binding at this target site by small sugar molecules, inducers, provides the basis for regulation. Different ligand binding activities are associated with the isolated domains of Lac I: specific and nonspecific DNA binding with the NH2 termini, and inducer and specific DNA binding with the core protein. This antibody could possibly be used to recover and purify protein/nucleic acid complexes (host and viral proteins associated with the viral DNA) from infected cells.

    Gen-ID

    8156885, 146576

    UniProt

    P03023
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