Biotin Antikörper (FITC)

Produktnummer ABIN349567

Hersteller Produkt- Nr.: 800-102-098

Produktdetails anti-Biotin Antikörper (FITC)

Target: Biotin

Fragment: Fab fragment

Reaktivität: Bitte anfragen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Biotin Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), Fluorescence Microscopy (FM), FLISA, Dot Blot (DB)

Hersteller Produkt- Nr.: 800-102-098

Hersteller: Rockland

Verwendungszweck: Fab Biotin Antibody Fluorescein Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Fluorescein.

Produktmerkmale: Synonyms: Goat Fab Anti-Biotin Antibody Fluorescein Conjugation, Goat Fab Anti-Biotin FITC Conjugated Antibody

Aufreinigung: Fab Biotin antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads followed by papain digestion and chromatographic separation.

Immunogen:

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Immunogen Type: Other

Isotyp: IgG

Labeling Ratio: 3.51

Anwendungsinformationen

Applikationshinweise:

Application Note: Fab Anti-Biotin Antibody Fluorescein Conjugated has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

FLISA Dilution: User Optimized

IF Microscopy Dilution: 1:500 - 1:2,500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu Biotin

Target: Biotin

Abstract: Biotin Produkte

Substanzklasse: Chemical

Hintergrund: Background: Fab Biotin antibody recognizes Biotin. Biotin, also known as vitamin H or coenzyme R, is a water-soluble B-vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis.