Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Threonine-X-Arginine in IHC (p), ELISA und WB. Es zeigt Reaktivität gegenüber Proben von Alle Spezies.
Kurzübersicht für Threonine-X-Arginine (phosphorylated) Antikörper (ABIN344518)
Target
Threonine-X-Arginine
Reaktivität
Alle Spezies
Wirt
Kaninchen
Klonalität
Polyklonal
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
Bindungsspezifität
phosphorylated
Spezifität
Detects endogenous levels of proteins containing the phospho-Thr-X-Arg motif. Detects phosphorylated Thr followed by Arg or Lys at the +2 position, though its reactivity is lower for the Lys compared to Arg at the +2 position. Does not cross-react with nonphospho-Thr or phospho-Ser in the same motif. It recognizes phospho-Thr in the FFT*R motif in PKCbeta II but does not recognize phospho-Thr in other motifs that lack Lys or Arg at +2. Recognizes all species.
Aufreinigung
Protein A Column
Immunogen
Synthetic peptide containing the phospho-Thr-X-Arg motif.
Isotyp
IgG
Applikationshinweise
ELISA (1:1000), IHC-P, WB (1:1000)
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.