Luciferase Antikörper

Produktnummer ABIN339711

Dieses Anti-Luciferase-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Luciferase in WB, IF, IHC und IHC (fro). Geeignet für Photinus pyralis.

Kurzübersicht für Luciferase Antikörper (ABIN339711)

Target: Luciferase

Reaktivität: Photinus pyralis

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Luciferase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Luciferase Antikörper

Spezifität: This antibody specifically reacts with luciferase protein, a reporter used to quantify expression levels from promoters in tissue culture and in vivo. Reactivity was confirmed via Western Blot analysis on the following samples containing added purified luciferase protein: mouse heart, liver, kidney, and cortex tissues. There was no cross reactivity to mouse heart, NIH3T3, Jurkat, and human fibroblasts. In IHC (frozen tissue) and Immunofluorescence applications, our antibody was able to detect tissue specific expression of Luciferase protein in Drosophila embryos using the well-characterized Gal4-UAS system with two different Gal4 drivers.

Aufreinigung: Protein A purified

Immunogen: Luciferase protein from Photinus pyralis.

Type of Immunogen: Purified protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: IF (10 - 30 μg/mL), IHC, IHC-Fr, WB (1 - 3 μg/mL)

Usage: Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence. Western Blot: 1-3 μg/mL. Observed in mouse heart, liver, kidney, and cortex tissues containing added purified luciferase protein. Immunohistochemistry (Frozen Tissue): 15-50 μg/mL. Detects tissue specific expression of Luciferase protein in Drosophila embryos using Gal4-UAS system with two different Gal4 drivers. Immunofluorescence: 10-30 μg/mL.

Kommentare:

Target Species of Antibody: Photinus pyralis

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Luciferase

Target: Luciferase

Andere Bezeichnung: Firefly Luciferase

Hintergrund: Synonyms: Firefly Luciferase, PPLUC

UniProt: P08659