HSF1 Antikörper

Produktnummer ABIN337399

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch HSF1 in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Kaninchen und Affe.

Kurzübersicht für HSF1 Antikörper (ABIN337399)

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reaktivität: Human, Maus, Ratte, Kaninchen, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-HSF1 Antikörper

Marke: IHC-plus™

Spezifität: Recognizes human HSF-1 at ~85kD protein in unstressed cells and an ~95kD protein in heat shocked cells, corresponding to the molecular masses of the inactive and activated forms respectively of HSF-1. Species cross-reactivity: mouse, rat, monkey, rabbit.

Aufreinigung: Protein A purified

Immunogen: Recombinant human HSF-1 expressed in E. coli.

Type of Immunogen: Recombinant protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1:500)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Positive control: Recombinant Human HSF-1 Protein.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Andere Bezeichnung: HSF1

Hintergrund: Name/Gene ID: HSF1

Synonyms: HSF1, HSTF 1, Heat shock factor protein 1, HSF 1, HSTF1

Gen-ID: 3297

UniProt: Q00613