PAR1 Antikörper (N-Term)

Produktnummer ABIN337370

Der Maus Monoklonal Anti-PAR1-Antikörper wurde für ELISA, IHC, FACS, IF und IHC (p) validiert. Er ist geeignet, PAR1 in Proben von Human zu detektieren.

Kurzübersicht für PAR1 Antikörper (N-Term) (ABIN337370)

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PAR1 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: ATAP2

Produktdetails anti-PAR1 Antikörper

Bindungsspezifität: N-Term

Marke: IHC-plus™

Spezifität: Recognizes the cleaved and intact forms of human thrombin receptor PAR1, N-terminus. Does not cross-react with PAR2.

Aufreinigung: Protein A purified

Immunogen: A synthetic peptide corresponding to the N-terminal region of the human thrombin receptor PAR1.

Type of Immunogen: Synthetic peptide

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.1 - 1 μg/mL), Flo (10 - 20 μg/mL), IF (1 - 3 μg/mL), IHC, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Immunofluorescence (Methanol or acetone fixed CHRF cells): 1-3 μg/mL. Flow Cytometry (Methanol or acetone fixed CHRF cells): 10-20 μg/mL. ELISA: 0.1-1.0 μg/mL.

Not recommended for: WB

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu PAR1

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Andere Bezeichnung: F2R / Thrombin Receptor / PAR1

Hintergrund: Name/Gene ID: F2R
Subfamily: Proteinase-activated
Family: GPCR

Synonyms: F2R, CF2R, Coagulation factor II receptor, PAR1, PAR-1, Thrombin receptor, TR, HTR, Protease-activated receptor 1

Gen-ID: 2149

UniProt: P25116

Pathways: Nuclear Receptor Transcription Pathway, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus