Transgelin Antikörper (AA 190-201)

Produktnummer ABIN337285

Dieser Ziege Polyklonal Antikörper detektiert spezifisch Transgelin in WB, IHC, ELISA und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Rind (Kuh), Schwein, Hund, Pferd, Hamster, Affe, Fledermaus und Xenopus laevis.

Kurzübersicht für Transgelin Antikörper (AA 190-201) (ABIN337285)

Target: Transgelin (TAGLN)

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein, Hund, Pferd, Hamster, Affe, Fledermaus, Xenopus laevis

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Transgelin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Transgelin Antikörper

Bindungsspezifität: AA 190-201

Spezifität: Human TAGLN / SM22. NP_003177.2 and NP_001001522.1 represent identical protein.

Homologie: Percent identity by BLAST analysis: Human, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Xenopus (100%) Stickleback (92%) Turkey, Chicken, Lizard, Catfish, Salmon, Pike, Zebrafish (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-MTGYGRPRQIIS from the C-terminus of human TAGLN / SM22 (NP_003177.2). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Xenopus (100%), Stickleback (92%), Turkey, Chicken, Lizard, Catfish, Salmon, Pike, Zebrafish (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:2000), WB (1:2000, 0 - 0 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Transgelin

Target: Transgelin (TAGLN)

Andere Bezeichnung: TAGLN / Transgelin / SM22

Hintergrund: Name/Gene ID: TAGLN

Synonyms: TAGLN, 22 kDa actin-binding protein, SMCC, TAGLN1, WS3-10, Transgelin, Transgelin variant 2, SM22, Protein WS3-10, SM22-alpha

Gen-ID: 6876

NCBI Accession: NP_003177

UniProt: Q01995