CLDN14 Antikörper (AA 227-239)

Produktnummer ABIN337255

Dieser Ziege Polyklonal Antikörper detektiert spezifisch CLDN14 in WB, ELISA, IHC und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Affe und Kaninchen.

Kurzübersicht für CLDN14 Antikörper (AA 227-239) (ABIN337255)

Target: CLDN14 (Claudin 14 (CLDN14))

Reaktivität: Human, Affe, Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CLDN14 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CLDN14 Antikörper

Bindungsspezifität: AA 227-239

Spezifität: Human CLDN14 / Claudin 14. Reported variants represent identical protein (NP_036262.1, NP_652763.1, NP_001139551.1, NP_001139550.1 and NP_001139549.1).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Rabbit (100%) Mouse, Rat, Hamster, Elephant, Bovine, Horse, Opossum, Chicken, Lizard (92%) Panda, Dog, Pig (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-SATHSGYRLNDYV from the C-terminus of human CLDN14 / Claudin 14 (NP_036262.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Rabbit (100%), Mouse, Rat, Hamster, Elephant, Bovine, Horse, Opossum, Chicken, Lizard (92%), Panda, Dog, Pig (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:128000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu CLDN14

Target: CLDN14 (Claudin 14 (CLDN14))

Andere Bezeichnung: CLDN14 / Claudin 14

Hintergrund: Name/Gene ID: CLDN14
Family: Claudin

Synonyms: CLDN14, Claudin 14, DFNB29, Claudin-14

Gen-ID: 23562

NCBI Accession: NP_036262

UniProt: O95500

Pathways: Cell-Cell Junction Organization, Hepatitis C