SKP2 Antikörper

Produktnummer ABIN337213

Dieses Maus Monoklonal-Antikörper erkennt spezifisch SKP2 in WB, ELISA, IHC, IP und IHC (p). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für SKP2 Antikörper (ABIN337213)

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SKP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-SKP2 Antikörper

Marke: IHC-plus™

Spezifität: Recognizes only human p45SKP2. Cross-reactivity with related endogenous proteins has not been observed.

Aufreinigung: Protein A purified

Immunogen: Recombinant, full-length human p45SKP2 protein.

Type of Immunogen: Recombinant protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (5 μg/mL), IP, WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Positive control: HeLa, Jurkat and 293T cell lines.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu SKP2

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Andere Bezeichnung: SKP2

Hintergrund: Name/Gene ID: SKP2

Synonyms: SKP2, F-box/LRR-repeat protein 1, FBL1, FLB1, F-box protein Skp2, FBXL1, p45, p45skp2

Gen-ID: 6502

UniProt: Q13309

Pathways: Mitotic G1-G1/S Phases