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DUSP1 Antikörper (C-Term)

DUSP1 Reaktivität: Human, Ratte, Hund WB, IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN337195
  • Target Alle DUSP1 Antikörper anzeigen
    DUSP1 (Dual Specificity Phosphatase 1 (DUSP1))
    Bindungsspezifität
    • 17
    • 15
    • 8
    • 7
    • 7
    • 5
    • 5
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 87-101, C-Term
    Reaktivität
    • 84
    • 34
    • 34
    • 1
    • 1
    • 1
    • 1
    Human, Ratte, Hund
    Wirt
    • 80
    • 3
    • 1
    Kaninchen
    Klonalität
    • 73
    • 11
    Polyklonal
    Konjugat
    • 28
    • 7
    • 6
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Dieser DUSP1 Antikörper ist unkonjugiert
    Applikation
    • 63
    • 37
    • 27
    • 26
    • 13
    • 10
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Recognizes human MKP1, Mr ~42kD. Species cross-reactivity: mouse. Predicted to cross-react with rat based on sequence homology.
    Homologie
    Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Rat, Dog (100%) Monkey, Mouse, Panda, Bovine, Rabbit (93%) Hamster, Pig (87%) Elephant (80%).
    Aufreinigung
    Protein A purified
    Immunogen
    KLH-conjugated, synthetic peptide corresponding to amino acids 87-101 (DERSAALDGAKRDGT-C) of human MAP kinase phosphatase 1 (MKP1), with a C-terminal cysteine added for conjugation purposes. The immunizing sequence is identical in rat and mouse. Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Rat, Dog (100%), Monkey, Mouse, Panda, Bovine, Rabbit (93%), Hamster, Pig (87%), Elephant (80%).

    Type of Immunogen: Synthetic peptide - KLH conjugated
    Isotyp
    IgG
    Top Product
    Discover our top product DUSP1 Primärantikörper
  • Applikationshinweise
    Approved: IHC, IHC-P (5 μg/mL), WB (0.1 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.1-1 μg/mL detects MKP1 in RIPA lysates from 3T3/A31 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-MKP1 (0.3 μg/mL).
    Kommentare

    Target Species of Antibody: Human

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, before the addition of glycerol to 30 %
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Target
    DUSP1 (Dual Specificity Phosphatase 1 (DUSP1))
    Andere Bezeichnung
    MKP-1 / DUSP1 (DUSP1 Produkte)
    Synonyme
    CL100 antikoerper, HVH1 antikoerper, MKP-1 antikoerper, MKP1 antikoerper, PTPN10 antikoerper, 3CH134 antikoerper, Mkp1 antikoerper, Ptpn16 antikoerper, DUSP1 antikoerper, U19515 antikoerper, erp antikoerper, mkp-1 antikoerper, cb269 antikoerper, sb:cb269 antikoerper, zgc:55920 antikoerper, id:ibd2586 antikoerper, wu:fd19g02 antikoerper, wu:fe50g09 antikoerper, XCL100 antikoerper, dual specificity phosphatase 1 antikoerper, dual specificity phosphatase 1 S homeolog antikoerper, DUSP1 antikoerper, Dusp1 antikoerper, dusp1 antikoerper, dusp1.S antikoerper
    Hintergrund
    Name/Gene ID: DUSP1
    Subfamily: Dual specificity MKP
    Family: Protein Phosphatase

    Synonyms: DUSP1, 3CH134, Dual specificity phosphatase 1, ERK-inactivating phosphatase, HVH1, MAP kinase phosphatase 1, VH1, PTPN10, CL100, MKP-1, MKP1
    Gen-ID
    1843
    UniProt
    P28562
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