DUSP1 Antikörper (Dual Specificity Phosphatase 1) (AA 87-101)

Details for Product anti-DUSP1 Antibody No. ABIN337195
Antigen
  • CL100
  • HVH1
  • MKP-1
  • MKP1
  • PTPN10
  • 3CH134
  • Mkp1
  • Ptpn16
  • DUSP1
  • U19515
  • erp
  • mkp-1
  • cb269
  • sb:cb269
  • zgc:55920
  • id:ibd2586
  • wu:fd19g02
  • wu:fe50g09
  • XCL100
  • dual specificity phosphatase 1
  • dual specificity phosphatase 1 S homeolog
  • DUSP1
  • Dusp1
  • dusp1
  • dusp1.S
Epitop
AA 87-101, C-Term
22
20
15
9
9
9
5
4
3
3
2
1
1
1
1
1
1
Reaktivität
Hund, Human, Ratte (Rattus)
160
75
75
8
5
4
4
3
1
1
1
1
Wirt
Kaninchen
132
22
9
Klonalität
Polyklonal
Konjugat
Dieser DUSP1 Antikörper ist unkonjugiert
10
6
6
6
6
6
6
6
6
3
3
3
2
2
2
2
2
Applikation
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
99
70
44
28
27
6
5
4
4
4
2
2
1
Optionen
Marke IHC-plus™
Immunogen KLH-conjugated, synthetic peptide corresponding to amino acids 87-101 (DERSAALDGAKRDGT-C) of human MAP kinase phosphatase 1 (MKP1), with a C-terminal cysteine added for conjugation purposes. The immunizing sequence is identical in rat and mouse. Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Rat, Dog (100%), Monkey, Mouse, Panda, Bovine, Rabbit (93%), Hamster, Pig (87%), Elephant (80%).

Type of Immunogen: Synthetic peptide - KLH conjugated
Isotyp IgG
Spezifität Recognizes human MKP1, Mr ~42kD. Species cross-reactivity: mouse. Predicted to cross-react with rat based on sequence homology.
Homologie Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Rat, Dog (100%) Monkey, Mouse, Panda, Bovine, Rabbit (93%) Hamster, Pig (87%) Elephant (80%).
Reinigung Protein A purified
Plasmids, Primers & others Plasmide, Primers & weitere DUSP1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung MKP-1 / DUSP1 (DUSP1 Antibody Abstract)
Hintergrund Name/Gene ID: DUSP1
Subfamily: Dual specificity MKP
Family: Protein Phosphatase

Synonyms: DUSP1, 3CH134, Dual specificity phosphatase 1, ERK-inactivating phosphatase, HVH1, MAP kinase phosphatase 1, VH1, PTPN10, CL100, MKP-1, MKP1
Gen-ID 1843
UniProt P28562
Applikations-hinweise Approved: IHC, IHC-P (5 μg/mL), WB (0.1 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.1-1 μg/mL detects MKP1 in RIPA lysates from 3T3/A31 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-MKP1 (0.3 μg/mL).
Kommentare

Target Species of Antibody: Human

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration Lot specific
Buffer 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, before the addition of glycerol to 30 %
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Bilder des Herstellers
Image no. 1 for anti-Dual Specificity Phosphatase 1 (DUSP1) (AA 87-101), (C-Term) antibody (ABIN337195) Human Placenta (formalin-fixed, paraffin-embedded) stained with DUSP1 antibody ABIN33...
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