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DUSP1 Antikörper (C-Term)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch DUSP1 in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Ratte und Hund.
Produktnummer ABIN337195

Kurzübersicht für DUSP1 Antikörper (C-Term) (ABIN337195)

Target

Alle DUSP1 Antikörper anzeigen
DUSP1 (Dual Specificity Phosphatase 1 (DUSP1))

Reaktivität

  • 86
  • 42
  • 34
  • 1
  • 1
  • 1
  • 1
Human, Ratte, Hund

Wirt

  • 83
  • 4
  • 1
Kaninchen

Klonalität

  • 76
  • 12
Polyklonal

Konjugat

  • 32
  • 5
  • 5
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Dieser DUSP1 Antikörper ist unkonjugiert

Applikation

  • 63
  • 32
  • 27
  • 26
  • 17
  • 10
  • 6
  • 6
  • 5
  • 3
  • 3
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Bindungsspezifität

    • 16
    • 15
    • 6
    • 6
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 87-101, C-Term

    Marke

    IHC-plus™

    Spezifität

    Recognizes human MKP1, Mr ~42kD. Species cross-reactivity: mouse. Predicted to cross-react with rat based on sequence homology.

    Homologie

    Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Rat, Dog (100%) Monkey, Mouse, Panda, Bovine, Rabbit (93%) Hamster, Pig (87%) Elephant (80%).

    Aufreinigung

    Protein A purified

    Immunogen

    KLH-conjugated, synthetic peptide corresponding to amino acids 87-101 (DERSAALDGAKRDGT-C) of human MAP kinase phosphatase 1 (MKP1), with a C-terminal cysteine added for conjugation purposes. The immunizing sequence is identical in rat and mouse. Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Rat, Dog (100%), Monkey, Mouse, Panda, Bovine, Rabbit (93%), Hamster, Pig (87%), Elephant (80%).

    Type of Immunogen: Synthetic peptide - KLH conjugated

    Isotyp

    IgG
  • Applikationshinweise

    Approved: IHC, IHC-P (5 μg/mL), WB (0.1 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.1-1 μg/mL detects MKP1 in RIPA lysates from 3T3/A31 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-MKP1 (0.3 μg/mL).

    Kommentare

    Target Species of Antibody: Human

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    Lot specific

    Buffer

    0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, before the addition of glycerol to 30 %

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Target

    DUSP1 (Dual Specificity Phosphatase 1 (DUSP1))

    Andere Bezeichnung

    MKP-1 / DUSP1

    Hintergrund

    Name/Gene ID: DUSP1
    Subfamily: Dual specificity MKP
    Family: Protein Phosphatase

    Synonyms: DUSP1, 3CH134, Dual specificity phosphatase 1, ERK-inactivating phosphatase, HVH1, MAP kinase phosphatase 1, VH1, PTPN10, CL100, MKP-1, MKP1

    Gen-ID

    1843

    UniProt

    P28562
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