PC Antikörper (AA 886-899)

Produktnummer ABIN337183

Dieser Ziege Polyklonal Antikörper detektiert spezifisch PC in WB, IHC, ELISA und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Schwein, Rind (Kuh), Affe, Hund, Pferd, Hamster, Kaninchen und Fledermaus.

Kurzübersicht für PC Antikörper (AA 886-899) (ABIN337183)

Target: PC (Pyruvate Carboxylase (PC))

Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh), Affe, Hund, Pferd, Hamster, Kaninchen, Fledermaus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PC Antikörper

Bindungsspezifität: AA 886-899

Spezifität: Human PC / Pyruvate Carboxylase. All reported variants (NP_000911.2, NP_001035806.1, NP_071504.2) represent identical protein.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig (100%) Goat, Stickleback, Pufferfish (93%) Opossum, Chicken, Xenopus, Zebrafish (86%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-KFKEVKKAYVEANQ from an internal region of human PC / Pyruvate Carboxylase (NP_000911.2, NP_001035806.1, NP_071504.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig (100%), Goat, Stickleback, Pufferfish (93%), Opossum, Chicken, Xenopus, Zebrafish (86%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:16000), WB (0.03 - 0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu PC

Target: PC (Pyruvate Carboxylase (PC))

Andere Bezeichnung: PC / Pyruvate Carboxylase

Hintergrund: Name/Gene ID: PC

Synonyms: PC, PCB, Pyruvate carboxylase, Pyruvic carboxylase

Gen-ID: 5091

NCBI Accession: NP_000911, NP_001035806, NP_071504

UniProt: P11498