PAX2A Antikörper (pSer393)

Produktnummer ABIN337176

Dieses Anti-PAX2A-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PAX2A in WB, ELISA, IHC und IHC (p). Geeignet für Human.

Kurzübersicht für PAX2A Antikörper (pSer393) (ABIN337176)

Target: PAX2A (Paired Box Gene 2a (PAX2A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PAX2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PAX2A Antikörper

Bindungsspezifität: pSer393

Marke: IHC-plus™

Spezifität: Recognizes human Pax2 protein when phosphorylated at Ser393. Based on amino acid sequence homology with Pax5 and Pax8, cross-reactivity with these proteins is likely. Does not react with purified, unphosphorylated GST-Pax2 proteins. Reacts with human HEK293 cell lysates and purified GST-Pax2 proteins phosphorylated by JNK. Species sequence Homology: mouse, chicken, zebra sh and frog - 100 % .

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic phospho-peptide derived from the sequence surrounding the phosphorylated Ser393 residue of mouse and human Pax2 (paired box protein 2).

Type of Immunogen: Synthetic phospho-peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (1:50), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:50. Western Blot: Analysis of JNK-stimulated (phosphorylated) fusion proteins identifies a single band at ~50kD. In Western Blot analysis of HEK293 cells, a thick band between ~42-45kD is observed, which may represent different human Pax2 isoforms and/or Pax8.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu PAX2A

Target: PAX2A (Paired Box Gene 2a (PAX2A))

Andere Bezeichnung: PAX2

Hintergrund: Name/Gene ID: PAX2

Synonyms: PAX2, Paired box homeotic gene 2, Paired box protein Pax-2, PAPRS, Paired box 2, Paired box gene 2

Gen-ID: 5076

UniProt: Q02962

Pathways: Carbohydrate Homeostasis, Stem Cell Maintenance, Tube Formation