STAT5 A/B Antikörper (pTyr694)

Produktnummer ABIN337157

Der Kaninchen Polyklonal Anti-STAT5 A/B-Antikörper wurde für WB, ELISA, IHC und IHC (p) validiert. Er ist geeignet, STAT5 A/B in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für STAT5 A/B Antikörper (pTyr694) (ABIN337157)

Target: STAT5 A/B

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser STAT5 A/B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-STAT5 A/B Antikörper

Bindungsspezifität: pTyr694

Marke: IHC-plus™

Spezifität: A BLAST analysis was used to suggest cross-reactivity with Stat5 alpha from human, mouse and rat based on 100 % homology with the immunizing sequence. Cross-reactivity with Stat5 alpha from other sources has not been determined.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5 alpha protein.

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:25000), IHC, IHC-P (20 - 40 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20-40 μg/mL. Western Blot: Expect a band approximately 91 kD in size corresponding to phosphorylated Stat5a protein in the appropriate cell lysate or extract. This phosphospecific polyclonal antibody reacts with mouse Stat5a pY694 and shows minimal reactivity by ELISA against the nonphosphorylated form of the immunizing peptide.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu STAT5 A/B

Target: STAT5 A/B

Andere Bezeichnung: STAT5A+B

Hintergrund: This antibody detects: STAT5 A+B