CBX5 Antikörper (AA 88-102)

Produktnummer ABIN337121

Der Ziege Polyklonal anti-CBX5 Antikörper wird verwendet zum Nachweis von CBX5 in Proben von Human, Rind (Kuh), Schwein, Pferd, Schaf und Affe. Er wurde validiert für WB, ELISA, IHC und IHC (p).

Kurzübersicht für CBX5 Antikörper (AA 88-102) (ABIN337121)

Target: CBX5 (Chromobox Homolog 5 (CBX5))

Reaktivität: Human, Rind (Kuh), Schwein, Pferd, Schaf, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CBX5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CBX5 Antikörper

Bindungsspezifität: AA 88-102

Spezifität: Human CBX5 / HP1. Variants (NP_036249.1, NP_001120793.1, NP_001120794.1) encode the same protein.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Sheep, Bovine, Horse, Pig (100%) Mouse, Rat, Hamster, Elephant, Panda, Dog, Rabbit (93%) Bat (87%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-NKRKSNFSNSADDIK from an internal region of human CBX5 / HP1 (NP_036249.1, NP_001120793.1, NP_001120794.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Sheep, Bovine, Horse, Pig (100%), Mouse, Rat, Hamster, Elephant, Panda, Dog, Rabbit (93%), Bat (87%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:128000), WB (1:128000, 0.03 - 0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu CBX5

Target: CBX5 (Chromobox Homolog 5 (CBX5))

Andere Bezeichnung: CBX5 / HP1 Alpha

Hintergrund: Name/Gene ID: CBX5

Synonyms: CBX5, Chromobox homolog 5, HP1Hs alpha, HP1Hs-alpha, HP1, HP1 alpha homolog, HP1 alpha, HP1-ALPHA, HP1A, Antigen p25, Chromobox protein homolog 5

Gen-ID: 23468

NCBI Accession: NP_036249, NP_001120793, NP_001120794

UniProt: P45973