F2RL1 Antikörper (N-Term)

Produktnummer ABIN337049

Der Maus Monoklonal Anti-F2RL1 Antikörper wurde für IHC, ELISA, IF und IHC (p) validiert. Er ist geeignet, F2RL1 in Proben von Human zu detektieren.

Kurzübersicht für F2RL1 Antikörper (N-Term) (ABIN337049)

Target: F2RL1 (Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser F2RL1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: SAM11

Produktdetails anti-F2RL1 Antikörper

Bindungsspezifität: N-Term

Spezifität: Recognizes native peptide and the N-terminus of the human PAR2 receptor. Does not cross-react with PAR1.

Aufreinigung: Protein A purified

Immunogen: Synthetic peptide corresponding to the N-terminal of the human PAR2 receptor.

Type of Immunogen: Synthetic peptide

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.1 - 1 μg/mL), IF (1 - 3 μg/mL), IHC, IHC-P (5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunofluorescence: 1-3 μg/mL. ELISA: 0.1-1 μg/mL. Western Blot: 1-3 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu F2RL1

Target: F2RL1 (Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1))

Andere Bezeichnung: F2RL1 / PAR2

Hintergrund: Name/Gene ID: F2RL1
Subfamily: Proteinase-activated
Family: GPCR

Synonyms: F2RL1, PAR2, Thrombin receptor-like 1, Protease-activated receptor 2, G-protein coupled receptor 11, GPR11, PAR-2

Gen-ID: 2150

UniProt: P55085

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, SARS-CoV-2 Protein Interaktom