Occludin Antikörper (C-Term)

Produktnummer ABIN337048

This anti-Occludin antibody (ABIN337048) is a Mouse Monoclonal antibody detecting Occludin in WB, ELISA, IHC, IF, IHC (p). Suitable for Human, Mouse, Rat, Dog.

Quick Overview for Occludin Antikörper (C-Term) (ABIN337048)

Target: Occludin (OCLN)

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Occludin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: OC-3F10

Produktdetails anti-Occludin Antikörper

Bindungsspezifität: C-Term

Marke: IHC-plus™

Spezifität: Recognizes mammalian Occludin Protein. Species cross-reactivity: human, mouse, rat and canine.

Aufreinigung: Protein A purified

Immunogen: GST fusion protein consisting of the C-terminal region (~150aa) of human occludin.

Type of Immunogen: Fusion protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.1 - 1 μg/mL), IF (2 - 3 μg/mL), IHC, IHC-P (10 μg/mL), WB (0.1 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Positive control: T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), rat liver and Caco-2 cells (human colon adenocarcinoma). Does not appear to detect the most highly phosphorylated form of the occludin protein when tested on Caco-2 cells.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Referenzen für anti-Occludin Antikörper (ABIN337048)

Krueger, Härtig, Reichenbach, Bechmann, Michalski: "Blood-brain barrier breakdown after embolic stroke in rats occurs without ultrastructural evidence for disrupting tight junctions." in: PLoS ONE, Vol. 8, Issue 2, pp. e56419, (2013) (PubMed).

Details zu Occludin

Target: Occludin (OCLN)

Andere Bezeichnung: OCLN / Occludin

Hintergrund: Name/Gene ID: OCLN

Synonyms: OCLN, Occludin, BLCPMG

Gen-ID: 100506658

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C