OB Cadherin Antikörper (Intracellular)

Produktnummer ABIN337036

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch OB Cadherin in WB, ELISA, IHC, IHC (fro) und IHC (p). Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für OB Cadherin Antikörper (Intracellular) (ABIN337036)

Target: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser OB Cadherin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-OB Cadherin Antikörper

Bindungsspezifität: Intracellular

Marke: IHC-plus™

Spezifität: Recognizes human Cadherin 11 at ~110kD. An additional band at ~130kD (presumably the Cadherin 11 precursor) may also be observed. Some crossreactivity with other Cadherins is observed in approximately the following order: Cadherin11>>R-Cadherin>Cadherin 8>>N-Cadherin=P-Cadherin. No crossreactivity with Cadherin 6 or E-Cadherin has been observed.

Aufreinigung: Immunoaffinity purified

Immunogen: Recombinant protein derived from the intracellular domain of the human Cadherin 11 protein.

Type of Immunogen: Recombinant protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-Fr, IHC-P (5 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Positive control: MDA. MB231 cells (human) and mouse L fibroblast lysates.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu OB Cadherin

Target: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Andere Bezeichnung: CDH11 / Cadherin 11

Hintergrund: Name/Gene ID: CDH11
Family: Cadherin

Synonyms: CDH11, CAD11, Cadherin 11, Cadherin-11, CDHOB, OSF-4, Osteoblast cadherin, OB, OB-cadherin, Ventral neural cadherin, Vnc

Gen-ID: 1009

UniProt: P55287