OB Cadherin Antikörper (Intracellular)

Produktnummer ABIN337035

Dieses Anti-OB Cadherin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von OB Cadherin in WB, ELISA, IHC, IP, IHC (fro) und IHC (p). Geeignet für Human und Maus.

Kurzübersicht für OB Cadherin Antikörper (Intracellular) (ABIN337035)

Target: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser OB Cadherin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 5B2H5

Produktdetails anti-OB Cadherin Antikörper

Bindungsspezifität: Intracellular

Marke: IHC-plus™

Spezifität: Recognizes the ~110kD human Cadherin 11 protein. Cross-reactivity with other cadherin family members has not been observed.

Aufreinigung: Protein A purified

Immunogen: Recombinant protein consisting of intracellular domain of human cadherin 11.

Type of Immunogen: Recombinant protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC, IHC-Fr (5 - 10 μg/mL), IHC-P (5 - 10 μg/mL), IP (5 μg/mL), WB (2 - 4 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5-10 μg/mL. Immunoprecipitation: 5 μg. Native, human, Not tested for mouse. Positive control: MDA. MB231 cells (human) and mouse L fibroblast lysates.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Aliquot to Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C (no longer than 1 week)
Long term -20°C
Aliquot to avoid freeze/thaw cycles.

Details zu OB Cadherin

Target: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Andere Bezeichnung: CDH11 / Cadherin 11

Hintergrund: Name/Gene ID: CDH11
Family: Cadherin

Synonyms: CDH11, CAD11, Cadherin 11, Cadherin-11, CDHOB, OSF-4, Osteoblast cadherin, OB, OB-cadherin, Ventral neural cadherin, Vnc

Gen-ID: 1009

UniProt: P55287