Midline 2 Antikörper (AA 723-735)

Produktnummer ABIN337007

Der Ziege Polyklonal anti-Midline 2 Antikörper wird verwendet zum Nachweis von Midline 2 in Proben von Human, Maus und Affe. Er wurde validiert für WB, ELISA, IHC und IHC (p).

Kurzübersicht für Midline 2 Antikörper (AA 723-735) (ABIN337007)

Target: Midline 2 (MID2)

Reaktivität: Human, Maus, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Midline 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Midline 2 Antikörper

Bindungsspezifität: AA 723-735

Spezifität: Human MID2. This antibody is expected to recognise both human isoforms of this protein.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%) Mouse, Rat, Dog, Bat, Elephant, Panda, Horse, Pig, Opossum, Turkey, Chicken (92%) Hamster, Rabbit, Zebrafish, Stickleback (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide QESPYVSGMKTCH from the C-terminus of human MID2 (NP_036348.2, NP_438112.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%), Mouse, Rat, Dog, Bat, Elephant, Panda, Horse, Pig, Opossum, Turkey, Chicken (92%), Hamster, Rabbit, Zebrafish, Stickleback (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Midline 2

Target: Midline 2 (MID2)

Andere Bezeichnung: TRIM1 / MID2

Hintergrund: Name/Gene ID: MID2

Synonyms: MID2, FXY2, Midin-2, Midline-2, Midin 2, Midline 2, Midline defect 2, RNF60, RING finger protein 60, TRIM1, Tripartite motif protein 1

Gen-ID: 11043

NCBI Accession: NP_036348, NP_438112

UniProt: Q9UJV3