S1PR3 Antikörper (AA 12-25)

Produktnummer ABIN337001

Der Kaninchen Polyklonal Anti-S1PR3-Antikörper wurde für WB, IHC, IHC (p) und ICC validiert. Er ist geeignet, S1PR3 in Proben von Human zu detektieren.

Kurzübersicht für S1PR3 Antikörper (AA 12-25) (ABIN337001)

Target: S1PR3 (Sphingosine-1-Phosphate Receptor 3 (S1PR3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser S1PR3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Produktdetails anti-S1PR3 Antikörper

Bindungsspezifität: AA 12-25

Marke: IHC-plus™

Spezifität: Recognizes human EDG 3 receptor at ~47kD in human cerebral cortex. Liver and heart tissues display 2-3 bands between 40-50kD, possibly due to different degrees of glycosylation.

Homologie: Percent identity by BLAST analysis: Human, Gorilla (100%) Gibbon (93%) Marmoset (86%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to aa12-25 [VRGNETLREHYQYV] of human EDG 3 protein. Species sequence Homology: Mouse, rat: 79% (11/14). Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon (93%), Marmoset (86%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IHC, IHC-P (10 μg/mL), WB (3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: TBS,  pH 7.4, 5 mg/mL BSA

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu S1PR3

Target: S1PR3 (Sphingosine-1-Phosphate Receptor 3 (S1PR3))

Andere Bezeichnung: SIPR3 / EDG3 / S1P3

Hintergrund: Name/Gene ID: S1PR3
Subfamily: Lysophospholipid/Lysosphingolipid
Family: GPCR

Synonyms: S1PR3, EDG-3, EDG3, LPB3, S1P receptor EDG3, S1P3, S1P receptor Edg-3, S1P receptor 3

Gen-ID: 1903

UniProt: Q99500