OCT4 Antikörper (AA 100-200)

Produktnummer ABIN336287

Der Kaninchen Polyklonal Anti-OCT4-Antikörper wurde für WB validiert. Er ist geeignet, OCT4 in Proben von Human, Maus und Protozoa zu detektieren.

Kurzübersicht für OCT4 Antikörper (AA 100-200) (ABIN336287)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reaktivität: Human, Maus, Protozoa

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser OCT4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-OCT4 Antikörper

Bindungsspezifität: AA 100-200

Kreuzreaktivität: Human, Maus, Primate

Aufreinigung: affinity purified

Immunogen: A synthetic peptide made to an internal portion of the human OCT4 protein sequence (between AA 100-200).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: -20 °C

Details zu OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Andere Bezeichnung: OCT-4

Hintergrund: Oct4, also known as POU domain transcription factor, Octomerbinding transcription factor 3 and Oct3, is expressed in human and mouse totipotent embryonic stem and germ cells. It may function in preventing these cells from differentiating. Each type of established mammalian pluripotent stem cell line (embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells) expresses Oct4, which disappears rapidly when the cells differentiate. Most studies on Oct4 have been performed on murine embryos. In human oocytes of various maturity and normally developing preimplantation/cleavage embryos, a variable Oct4 expression pattern has been revealed, concomitant with a pure cytoplasmic localization of the protein. This variability in expression fades away during compaction, indicating embryonic Oct4 expression, the protein appears in the nucleus, implying biological activity. Functional Oct4 has been found in human amniotic fluid. As Oct4 transcripts have also been found in various differentiated cells, the presence of Oct-4 transcripts or proteins may not be sufficient for identifying undifferentiated cell lines in humans. It may also be important to examine the localization of Oct4 proteins within a cell. Oct4 has been shown to interact with Nanog and with proteins from multiple repression complexes, including the NuRD, Sin3A and Pml complexes, possibly to control ES fate. Reactivation of Oct4 expression is postulated to occur in differentiated cells that have undergone carcinogenesis, or tumor formation

Pathways: Stem Cell Maintenance