OB Cadherin Antikörper

Produktnummer ABIN335403

Dieses Anti-OB Cadherin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von OB Cadherin in WB, IHC, ICC und IHC (fro). Geeignet für Human und Ratte. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für OB Cadherin Antikörper (ABIN335403)

Target: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser OB Cadherin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 16A

Produktdetails anti-OB Cadherin Antikörper

Spezifität: Human and rat.

Aufreinigung: Protein A affinity column

Immunogen: 16A is a mouse monoclonal IgG1 antibody obtained by fusion of SP2/0 mouse myeloma cells with spleen cells from a mouse immunized with affinity purified extracellular domain of human cadherin-11-GST fusion protein.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: 16A recognizes the extracellular domain of cadherin-11. 16A is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen sections when using a PBS buffer containing 0.1 mM CaCl 2 and 0.1 mM MgCl 2 . Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:50 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:25 - 1:250 for immunoblotting applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Referenzen für anti-OB Cadherin Antikörper (ABIN335403)

Tomita, van Bokhoven, van Leenders, Ruijter, Jansen, Bussemakers, Schalken: "Cadherin switching in human prostate cancer progression." in: Cancer research, Vol. 60, Issue 13, pp. 3650-4, (2000) (PubMed).

Bussemakers, Van Bokhoven, Tomita, Jansen, Schalken: "Complex cadherin expression in human prostate cancer cells." in: International journal of cancer. Journal international du cancer, Vol. 85, Issue 3, pp. 446-50, (2000) (PubMed).

Details zu OB Cadherin

Target: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Andere Bezeichnung: OB-Cadherin / Cadherin-11

Hintergrund: Cadherins constitute a family of transmembrane glycoproteins involved in Ca 2+ -dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. The progression of carcinomas is associated with the loss of epithelial morphology and a concomitant acquisition of a more mesenchymal phenotype, which is thought to contribute to the invasive and/or metastatic behavior. A putative role for cadherin-11 in these late stages of tumor progression is based on the fact that migration of mesenchymal cells is facilitated when cadherin-11 is highly expressed.