Selectin E/CD62e Antikörper

Produktnummer ABIN335384

Der Ratte Monoklonal Anti-Selectin E/CD62e-Antikörper wurde für WB, IHC, ELISA, IP, FACS, ICC, IHC (fro) und Func validiert. Er ist geeignet, Selectin E/CD62e in Proben von Maus zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für Selectin E/CD62e Antikörper (ABIN335384)

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Selectin E/CD62e Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Klon: UZ5

Produktdetails anti-Selectin E/CD62e Antikörper

Spezifität: Mouse.

Aufreinigung: Purified

Immunogen: UZ5 is a rat monoclonal IgG2b antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a Lewis rat immunized with LPS-activated mlEND1 cells expressing E-selectin at their cell surface.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: UZ5 recognizes a conformational epitope in the CR1 region in murine E-selectin. UZ5 partially inhibits leukocyte adhesion to murine activated endothelial cells. UZ5 is suitable for immunoblotting, ELISA, immunoprecipitation, immunohistochemistry on fozen sections and flow cytometry (dilution 1:200).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Referenzen für anti-Selectin E/CD62e Antikörper (ABIN335384)

Hammel, Weitz-Schmidt, Krause, Moll, Vestweber, Zerwes, Hallmann: "Species-specific and conserved epitopes on mouse and human E-selectin important for leukocyte adhesion." in: Experimental cell research, Vol. 269, Issue 2, pp. 266-74, (2001) (PubMed).

Hallmann, Zimmermann, Sorokin, Needham, Von der Mark: "Adhesion of leukocytes to the inflammed endothelium." in: Scandinavian journal of rheumatology. Supplement, Vol. 101, pp. 107-9, (1995) (PubMed).

Details zu Selectin E/CD62e

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Andere Bezeichnung: E-selectin

Hintergrund: Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkappaB dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.

Pathways: Thromboxane A2 Receptor Signaling