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Vimentin Antikörper

Name: Vimentin Antikörper
Brand: antibodies-online
SKU: ABIN335382
Price: 637.79 EUR
Availability: InStock

VIM Reaktivität: Human, Ratte, Huhn, Potoroo WB, IHC, FACS, IHC (p), ICC, IHC (fro) Wirt: Maus Monoclonal V9 unconjugated

Image no. 2 for anti-Vimentin (VIM) antibody (ABIN335382) (Vimentin Antikörper)

Image no. 1 for anti-Vimentin (VIM) antibody (ABIN335382) (Vimentin Antikörper)

Image no. 3 for anti-Vimentin (VIM) antibody (ABIN335382) (Vimentin Antikörper)

3 Abbildungen

(6 Referenzen)

Produktnummer ABIN335382

Datenblatt (PDF)

Target Alle Vimentin (VIM) Antikörper anzeigen

Vimentin (VIM)
Reaktivität
320

129

126

61

60

50

42

38

30

22

20

14

13

8

8

6

6

3

3

1

1

1

1

1

1

1

1

1

1
Human, Ratte, Huhn, Potoroo
Wirt
169

132

11

6

6

5

2

2
Maus
Passende Sekundärantikörper
Klonalität
183

149
Monoklonal
Konjugat
207

15

13

12

9

7

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

1

1
Dieser Vimentin Antikörper ist unkonjugiert
Applikation
271

117

110

97

92

63

46

40

39

32

23

23

8

6

4

4

3

2

2

2

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität

Human, rat, chicken and potoroo.

Aufreinigung

Purified

Immunogen

V9 is a mouse monoclonal IgG1 antibody derived by fusion of PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with vimentin isolated from porcine lens.

Klon

V9

Isotyp

IgG1

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VIM Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh), Affe WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Wirt: Kaninchen Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reaktivität: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody
VIM Reaktivität: Human, Maus, Ratte WB, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Säugetier WB, FACS, IHC (p), ICC Wirt: Maus Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Human, Maus, Ratte, Schwein, Huhn WB, IHC (p), ICC, IP Wirt: Maus Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Human, Maus WB, IF, FACS, IHC (p) Wirt: Kaninchen Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reaktivität: Human WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reaktivität: Human WB, IHC, ICC, IP Wirt: Maus Monoclonal C8 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reaktivität: Human WB, IF, FACS, IHC (p) Wirt: Kaninchen Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) (AA 41-140) antibody
VIM Reaktivität: Human WB, ELISA, IF, IP Wirt: Maus Monoclonal 3E9 unconjugated

Applikationshinweise

V9 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. V9 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues and flow cytometry. For staining paraffin-embedded tissues pretreatment by boiling tissue sections for 10 minutues in 10 mM citrate buffer (pH 6.0) is required. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Lagerung

4 °C
De Visscher, Plusquin, Mesure, Flameng: "Selection of an immunohistochemical panel for cardiovascular research in sheep." in: Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry, Vol. 18, Issue 4, pp. 382-91, (2010) (PubMed).

Corver, Koopman, van der Aa, Regensburg, Fleuren, Cornelisse: "Four-color multiparameter DNA flow cytometric method to study phenotypic intratumor heterogeneity in cervical cancer." in: Cytometry, Vol. 39, Issue 2, pp. 96-107, (2000) (PubMed).

Van Muijen, Ruiter, Warnaar: "Coexpression of intermediate filament polypeptides in human fetal and adult tissues." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 57, Issue 4, pp. 359-69, (1987) (PubMed).

Van Muijen, Warnaar, Ponec: "Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn, and adult epidermis." in: Experimental cell research, Vol. 171, Issue 2, pp. 331-45, (1987) (PubMed).

Tölle, Weber, Osborn: "Microinjection of monoclonal antibodies specific for one intermediate filament protein in cells containing multiple keratins allow insight into the composition of particular 10 nm filaments." in: European journal of cell biology, Vol. 38, Issue 2, pp. 234-44, (1985) (PubMed).

Osborn, Debus, Weber: "Monoclonal antibodies specific for vimentin." in: European journal of cell biology, Vol. 34, Issue 1, pp. 137-43, (1984) (PubMed).
Target

Vimentin (VIM)

Andere Bezeichnung

Vimentin (VIM Produkte)

Hintergrund

Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP, often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type.

Pathways

Caspase Kaskade in der Apoptose