Vimentin Antikörper

Produktnummer ABIN335381

Der Maus Monoklonal Anti-Vimentin-Antikörper wurde für WB, IHC, FACS, ICC und IHC (fro) validiert. Er ist geeignet, Vimentin in Proben von Human, Ratte, Schwein, Hund, Ziege und Hamster zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für Vimentin Antikörper (ABIN335381)

Target: Vimentin (VIM)

Reaktivität: Human, Ratte, Schwein, Hund, Ziege, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Vimentin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: RV203

Produktdetails anti-Vimentin Antikörper

Spezifität: Human, rat, goat, canine, hamster and swine.

Aufreinigung: Purified

Immunogen: RV203 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: RV203 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. RV203 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Referenzen für anti-Vimentin Antikörper (ABIN335381)

Schaart, Pieper, Kuijpers, Bloemendal, Ramaekers: "Baby hamster kidney (BHK-21/C13) cells can express striated muscle type proteins." in: Differentiation; research in biological diversity, Vol. 46, Issue 2, pp. 105-15, (1991) (PubMed).

Details zu Vimentin

Target: Vimentin (VIM)

Andere Bezeichnung: Vimentin

Hintergrund: Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.

Pathways: Caspase Kaskade in der Apoptose