CD56 Antikörper

Produktnummer ABIN335374

Produktdetails anti-CD56 Antikörper

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Reaktivität: Human, Zebrafisch (Danio rerio)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD56 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Spezifität: Human.

Aufreinigung: Purified

Immunogen: 123C3 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with the a membrane preparation of a small cell lung carcinoma specimen.

Klon: 123C3

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: 123C3 was defined as a cluster I antibody during the First International Workshop on Small Cell Lung Cancer (SCLC) Antibodies. 123C3 recognizes an epitope in the NCAM exons 11-13 which is dependent on an intact conformation of the first fibronectin type-III homologous domain encoded by these exons. 123C3 stains NCAM which is present in small cell lung cancer and lung carcinoids. It also reacts with a number of non-small cell lung carcinomas and neuroendocrine and neuronal derived tissues. In addition, 123C3 is internalized after binding to its antigen on SCLC cell lines, making it an excellent reagent for tumor imaging in xenograft models. 123C3 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Referenzen für anti-CD56 Antikörper (ABIN335374)

Kwa, Verheijen, Litvinov, Dijkman, Mooi, Van Krieken: "Prognostic factors in resected non-small cell lung cancer: an immunohistochemical study of 39 cases." in: Lung cancer (Amsterdam, Netherlands), Vol. 16, Issue 1, pp. 35-45, (1997) (PubMed).

Kwa, Verhoeven, Storm, van Zandwijk, Mooi, Hilkens: "Radioimmunotherapy of small-cell lung cancer xenografts using 131I-labelled anti-NCAM monoclonal antibody 123C3." in: Cancer immunology, immunotherapy : CII, Vol. 41, Issue 3, pp. 169-74, (1995) (PubMed).

Gerardy-Schahn, Eckhardt: "Hot spots of antigenicity in the neural cell adhesion molecule NCAM." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 8, pp. 38-42, (1994) (PubMed).

Kibbelaar, Moolenaar, Michalides, Van Bodegom, Vanderschueren, Wagenaar, Dingemans, Bitter-Suermann, Dalesio, Van Zandwijk: "Neural cell adhesion molecule expression, neuroendocrine differentiation and prognosis in lung carcinoma." in: European journal of cancer (Oxford, England : 1990), Vol. 27, Issue 4, pp. 431-5, (1991) (PubMed).

Moolenaar, Muller, Schol, Figdor, Bock, Bitter-Suermann, Michalides: "Expression of neural cell adhesion molecule-related sialoglycoprotein in small cell lung cancer and neuroblastoma cell lines H69 and CHP-212." in: Cancer research, Vol. 50, Issue 4, pp. 1102-6, (1990) (PubMed).

Schol, Mooi, van der Gugten, Wagenaar, Hilgers: "Monoclonal antibody 123C3, identifying small cell carcinoma phenotype in lung tumours, recognizes mainly, but not exclusively, endocrine and neuron-supporting normal tissues." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 2, pp. 34-40, (1988) (PubMed).

Mooi, Wagenaar, Schol, Hilgers: "Monoclonal antibody 123C3 in lung tumour classification. Immunohistology of 358 resected lung tumours." in: Molecular and cellular probes, Vol. 2, Issue 1, pp. 31-7, (1988) (PubMed).

Details zu CD56

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Andere Bezeichnung: NCAM / CD56 (NCAM1 Produkte)

Synonyme: CD56 antikoerper, MSK39 antikoerper, NCAM antikoerper, fj56g09 antikoerper, ncam antikoerper, ncam1 antikoerper, wu:fj56g09 antikoerper, NCAM1 antikoerper, n-cam antikoerper, E-NCAM antikoerper, NCAM-1 antikoerper, Ncam antikoerper, Cd56 antikoerper, N-CAM antikoerper, N-CAM-1 antikoerper, NCAM-C antikoerper, NCAMC antikoerper, N-CAM-1-B antikoerper, NCAM-1-B antikoerper, ncam1-a antikoerper, ncam1-b antikoerper, neural cell adhesion molecule 1 antikoerper, neural cell adhesion molecule 1a antikoerper, neural cell adhesion molecule 1 S homeolog antikoerper, neural cell adhesion molecule 1 L homeolog antikoerper, NCAM1 antikoerper, ncam1a antikoerper, ncam1 antikoerper, LOC100085373 antikoerper, ncam1.S antikoerper, Ncam1 antikoerper, ncam1.L antikoerper

Hintergrund: NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig)-like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins.