NEFH Antikörper

Produktnummer ABIN335369

Der Maus Monoklonal Anti-NEFH-Antikörper wurde für WB, IHC, IHC (p) und IHC (fro) validiert. Er ist geeignet, NEFH in Proben von Human, Ratte, Maus, Huhn, Rind (Kuh), Kaninchen, Meerschweinchen, Hund, Schaf, Xenopus laevis, Affe und Hamster zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für NEFH Antikörper (ABIN335369)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reaktivität: Human, Ratte, Maus, Huhn, Rind (Kuh), Kaninchen, Meerschweinchen, Hund, Schaf, Xenopus laevis, Affe, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NEFH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: RNF402

Produktdetails anti-NEFH Antikörper

Spezifität: Human, rat, rabbit, dog, hamster, chicken, mouse, cow, monkey, sheep, guinea pig and Xenopus.

Aufreinigung: Purified

Immunogen: RNF402 is a mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a neurofilament preparation of calf brain tissue.

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: RNF402 reacts with both the phosphorylated and non-phosphorylated isoform of the 200 kD neurofilament protein. RNF402 is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:10 - 1:100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:25 - 1:250 for immunoblotting applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Referenzen für anti-NEFH Antikörper (ABIN335369)

Bauwens, De Groot, Ramaekers, Veldman, Huizing: "Expression of intermediate filament proteins in the adult human vestibular labyrinth." in: The Annals of otology, rhinology, and laryngology, Vol. 101, Issue 6, pp. 479-86, (1992) (PubMed).

Kuijpers, Tonnaer, Peters, Ramaekers: "Expression of intermediate filament proteins in the mature inner ear of the rat and guinea pig." in: Hearing research, Vol. 52, Issue 1, pp. 133-46, (1991) (PubMed).

Details zu NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Andere Bezeichnung: Neurofilament 200 kDa

Hintergrund: Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.