Cardiotin Antikörper

Produktnummer ABIN335364

Der Maus Monoklonal anti-Cardiotin Antikörper (Klon SR-4) (ABIN335364) detektiert spezifisch Cardiotin in WB, IHC (fro) und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Schwein.

Kurzübersicht für Cardiotin Antikörper (ABIN335364)

Target: Cardiotin

Reaktivität: Human, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC)

Klon: SR-4

Produktdetails

Spezifität: Human and swine.

Aufreinigung: Purified

Immunogen: SR-4 is a mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with cardiotin.

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: SR-4 reacts with cardiotin, a mitochondrion-associated protein, which is present in cardiomyocytes and skeletal muscle, but also in epithelial cells and tissues. SR-4 is useful for immunohistochemistry on frozen tissue sections and immunoblotting. In immunoblotting assays SR-4 reacts exclusively with the 300 kDa cardiotin protein complex. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:50 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:25 - 1:250 for immunoblotting applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Antigendetails

Target: Cardiotin

Hintergrund: Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle alpha-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.