Ascorbate Oxidase Antikörper (Biotin)

Produktnummer ABIN334591

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Ascorbate Oxidase in ELISA und WB. Es zeigt Reaktivität gegenüber Proben von Cucurbita pepo.

Kurzübersicht für Ascorbate Oxidase Antikörper (Biotin) (ABIN334591)

Target: Ascorbate Oxidase (AO)

Reaktivität: Cucurbita pepo

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ascorbate Oxidase Antikörper ist konjugiert mit Biotin

Applikation: ELISA, Western Blotting (WB)

Produktdetails anti-Ascorbate Oxidase Antikörper (Biotin)

Verwendungszweck: Anti-AO | ascorbate oxidase, Biotin conjugated

Kreuzreaktivität (Details): Cucurbita sp.
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Glycne max,Oryza sativa

Aufreinigung: Purified IgG

Immunogen: ascorbate oxidase purified from Cucurbita sp.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: 1 : 1000-1 : 30 000 (ELISA), 1 : 1000 (WB)

Kommentare:

IgG concentration is 10 mg/ml. Biotin/IgG protein molar ratio is approximately 5.6. No foreign proteins are added.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 1 mL of sterile water, Allow reconstituted product to stand for at least 30 minutes at room temperature prior to dilution, If necessary, centrifuge to remove any particulates, Prepare fresh working dilution daily

Buffer: Purified IgG in PBS pH 7.2.

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working dilutions should be stored at 4°C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation, It will not affect the performance of the product.

Details zu Ascorbate Oxidase

Target: Ascorbate Oxidase (AO)

Hintergrund:

Background: Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90 % of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.

Additional information: IgG protein fraction is prepared by ammonium sulphate precipitation and ion exchanged chromatography, N-Hydroxysuccinimidobiotin is used for labelling of antibody

Molekulargewicht: 61 | 45 kDa (Cucurbita pepo)