HSP70 Antikörper (Cytoplasmic)

Produktnummer ABIN334584

Dieses Anti-HSP70-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von HSP70 in WB und IP. Geeignet für Plasmodium falciparum, Arabidopsis thaliana, Mais (Zea mays), Tomato, Gerste und Algae (Desmodesmus subspicatus). Dieses Primary Antibody wurde in 4+ Publikationen zitiert.

Kurzübersicht für HSP70 Antikörper (Cytoplasmic) (ABIN334584)

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Reaktivität: Plasmodium falciparum, Arabidopsis thaliana, Mais (Zea mays), Tomato, Gerste, Algae (Desmodesmus subspicatus)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSP70 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-HSP70 Antikörper

Bindungsspezifität: Cytoplasmic

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 70 kDa
Detection limit is 4 pg, 11 fmol, linear range of logit/log plot is 0.02-6 pmol,midrange (B/ B o =50 %) 112 pg, 300 fmol.

Aufreinigung: serum

Immunogen: KLH-conjugated synthetic peptide conserved in known higher plant HSC70 proteins including three isoforms of Arabidopsis thaliana HSC70-1 (NP_ 001119156.1), HSC70-2 (NP_195869.1) and HSC70-3 (NP_187555.1) as well as heat shock inducible Hsp70 of Arabidopsis thaliana At3g12580/T2E22_110 and At3g16030 and At3g12580/T2E22_110

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: 1 : 3000 with standard ECL on 5 µg of protein per well (WB), 2-3 µl/protein extractof concentration 3-5 mg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 150 µL of sterile water.

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Referenzen für anti-HSP70 Antikörper (ABIN334584)

Norhayati, Hazlina, Asrenee, Emilin: "Magnitude and risk factors for postpartum symptoms: a literature review." in: Journal of affective disorders, Vol. 175, pp. 34-52, (2015) (PubMed).

Muralidharan, Oksman, Pal, Lindquist, Goldberg: "Plasmodium falciparum heat shock protein 110 stabilizes the asparagine repeat-rich parasite proteome during malarial fevers." in: Nature communications, Vol. 3, pp. 1310, (2012) (PubMed).

Alvarez, del Hoyo, García-Breijo, Reig-Armiñana, del Campo, Guéra, Barreno, Casano: "Different strategies to achieve Pb-tolerance by the two Trebouxia algae coexisting in the lichen Ramalina farinacea." in: Journal of plant physiology, Vol. 169, Issue 18, pp. 1797-806, (2012) (PubMed).

Janicka-Russak, Kaba?a, Burzynski: "Different effect of cadmium and copper on H+-ATPase activity in plasma membrane vesicles from Cucumis sativus roots." in: Journal of experimental botany, Vol. 63, Issue 11, pp. 4133-42, (2012) (PubMed).

Details zu HSP70

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Andere Bezeichnung: HSP70

Hintergrund: AGI Code: At3g12580
Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.

Molekulargewicht: 70 kDa

NCBI Accession: NP_187555, NP_195869

UniProt: Q9LHA8