50S Ribosomal Protein L30 Antikörper

Produktnummer ABIN7676204

Der Kaninchen Polyklonal anti-50S Ribosomal Protein L30 Antikörper (ABIN7676204) detektiert spezifisch 50S Ribosomal Protein L30 in IF und WB. Dieser Antikörper reagiert spezifisch mit Proben aus Chlamydomonas reinhardtii und Spinach.

Kurzübersicht für 50S Ribosomal Protein L30 Antikörper (ABIN7676204)

Target: 50S Ribosomal Protein L30

Reaktivität: Chlamydomonas reinhardtii, Spinach

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Immunofluorescence (IF), Western Blotting (WB)

Produktdetails

Verwendungszweck: Anti-L7/L12 | Ribosomal protein

Kreuzreaktivität (Details): Chlamydomonas reinhardtii, weakly reacts with the r-protein in spinach ca. 1 %
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Aufreinigung: Serum

Immunogen: Purified native Chlamydomonas reinhardtii L-30 protein eluted from a gel piece

Anwendungsinformationen

Applikationshinweise: 1: 10 (IF), 1 : 1000 (WB)

Kommentare:

Cross react with L2 and L26 proteins of Chlamydomonas reinhardtii. L7/L12 is very acidic, may not bind well to nitrocellulose membrane and can have abberant mobility depending upon conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Antigendetails

Target: 50S Ribosomal Protein L30

Andere Bezeichnung: 50S ribosomal protein L30 (L-30)

Hintergrund:

Background: Ribosomal protein L-30 is a part of 50S ribosomal large subunit, synthesized in chloroplast. Schmidt et al. (1983) Sites of synthesis of chloroplast ribosomal proteins in Chlamydomonas. J Cell Biol 96(5):1451-1463

Additional information: Name of this antibody has been changed from L-30 | 50S ribosomal protein L30 to L7/L12 based on the following reference: Randolph-Anderson et al. (1989). Electrophoretic and immunological comparisons of chloroplast and prokaryotic ribosomal proteins reveal that certain families of large subunit proteins are evolutionarily conserved. J Mol Evol. 1989 Jul,29(1):68-88.

Molekulargewicht: 11.9 kDa (Chlamydomonas reinhardtii), 15.7 kDa (spinach)