ATP Synthase Subunit gamma (AtpC) Antikörper

Produktnummer ABIN334563

Dieses Anti--Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von in WB. Geeignet für Arabidopsis thaliana und Chlamydomonas reinhardtii.

Kurzübersicht für ATP Synthase Subunit gamma (AtpC) Antikörper (ABIN334563)

Target: ATP Synthase Subunit gamma (AtpC)

Reaktivität: Arabidopsis thaliana, Chlamydomonas reinhardtii

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails

Kreuzreaktivität (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Homologie: Glycine max, Lens culinaris, Nicotiana tabacum, Physcomitrella patens, Pisum sativum, Populus jackii, Vitis vinifera, cyanobacteria

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 42 kDa (Chlamydomonas reinhardtii), 38 kDa (Spinacia oleracea)

Aufreinigung: serum

Immunogen: synthetic peptides derived from Arabidopsis thalina chloroplast localized ATP synthase subunit gamma chain 1 and 2 protein sequence ( At4g04640 and At1g15700) and Chlamydomonas reinhardtii ATP synthase subunit gamma protein sequence ( A8HXL8) coupled to KLH

Anwendungsinformationen

Applikationshinweise: 1: 10 000 (WB), 1: 1000 (ELISA)

Kommentare:

apparent molecular weight of subunit gamma (and as general rule most of ATP synthase subunits) is quite different between Chlamydomonas (42 kDa) and higher plants (38 kDa in spinach), see figure in Lemaire et al. (1989)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 μL of sterile water

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Antigendetails

Target: ATP Synthase Subunit gamma (AtpC)

Andere Bezeichnung: Gamma subunit of ATP synthase (AtpC)

Hintergrund: AGI Code: At4g04640
ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex. Alternative name of gamma subunit is also: F-ATPase gamma subunit.

Molekulargewicht: expected: 35.3 , 42 (Chlamydomonas reinhardtii)35.6 kDa, apparent: 38 (Spinacia oleracea)

UniProt: Q01908, Q01909

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process