CDJ1 Antikörper (N-Term)

Produktnummer ABIN334548

Produktdetails anti-CDJ1 Antikörper

Target: CDJ1 (Chloroplast DnaJ Protein (CDJ1))

Bindungsspezifität: N-Term

Reaktivität: Chlamydomonas reinhardtii

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Immunoprecipitation (IP), Western Blotting (WB)

Kreuzreaktivität (Details): Not reactive in: higher plants

Homologie: Ostreococcus sp.

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 40 / 40 kDa

Aufreinigung: serum

Immunogen: fusion protein encoding region for the Chlamydomonas rerinhardtii N-terminal part of CDJ1 Q66YD3, overexpressed in E.coli

Anwendungsinformationen

Applikationshinweise: 1. 3000 with standard ECL (WB)

Kommentare:

western blot has been done on 10-50 µg load per well, immunoprecipitation has been done on 5-50 µl

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 μL of sterile water.

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Referenzen für anti-CDJ1 Antikörper (ABIN334548)

Long, Sommer, Allen, Lu, Merchant: "FER1 and FER2 encoding two ferritin complexes in Chlamydomonas reinhardtii chloroplasts are regulated by iron." in: Genetics, Vol. 179, Issue 1, pp. 137-47, (2008) (PubMed).

Willmund, Dorn, Schulz-Raffelt, Schroda: "The chloroplast DnaJ homolog CDJ1 of Chlamydomonas reinhardtii is part of a multichaperone complex containing HSP70B, CGE1, and HSP90C." in: Plant physiology, Vol. 148, Issue 4, pp. 2070-82, (2008) (PubMed).

Details zu CDJ1

Target: CDJ1 (Chloroplast DnaJ Protein (CDJ1))

Andere Bezeichnung: CDJ1

Synonyme: chloroplast DnaJ-like protein antikoerper, CDJ1 antikoerper

Hintergrund: CDJ1 is one of three zinc-finger containing J domain proteins of Chlamydomonas reinhardtii. This protein is found in chloroplast. In spite that CDJ1 gene is heat shock inducible it does not reflect in increased CDJ1 protein levels. CDJ1 forms complexes with plastidic HSP90C,HSP70B and CGE1 and is a new cohort protein of the chloroplast multi-chaperone complex.DnaJ/Hsp40 (heat shock protein 40) proteins are highly conserved and play crucial roles in protein translation, folding, unfolding, translocation, and degradation.

Molekulargewicht: expected: 40 kDa, apparent: 40 kDa

UniProt: Q66YD3