TOP2 Antikörper (C-Term, N-Term)

Produktnummer ABIN334538

Der Kaninchen Polyklonal Anti-TOP2-Antikörper wurde für WB und IL validiert. Er ist geeignet, TOP2 in Proben von Arabidopsis thaliana und Vicia faba zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für TOP2 Antikörper (C-Term, N-Term) (ABIN334538)

Target: TOP2 (DNA Topoisomerase II (TOP2))

Reaktivität: Arabidopsis thaliana, Vicia faba

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TOP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunolocalization (IL)

Produktdetails anti-TOP2 Antikörper

Bindungsspezifität: C-Term, N-Term

Spezifität: Antibody detects a protein of ca. 170 kDa on western blots of Arabidopsis thaliana protein extracts. In subcellular fractions of cultured Arabidopsis cells the antibody detects a 170 kDa protein exclusively in the nulear fraction.

Kreuzreaktivität (Details): Not reactive in: Nicotiana tabacum

Homologie: Brassica rapa, Chlamydomonas reinhardtii, Chlorella vulgaris, Citrus clementina, Glycine max, Hordeum vulgare, Medicago truncatula, Oryza sativa, Ostreococcus tauri, Panicum italicum, Phaseolus vulgaris, Physcomitrella patens, Pinus sitchensis, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Vitis vinifera, Volvox caterii

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 164 / 170 kDa (Arabidopsis thaliana)

Aufreinigung: serum

Immunogen: The C-terminal 153 amino acids of the Arabidopsis thaliana Topoisomerase II (At3g23890, protein accesion number P30182) with an N-terminal hexahistidine tag was expressed in E.coli and purified by Ni2+ affinity chromatography.

Anwendungsinformationen

Applikationshinweise: 1: 2000 (WB), 1: 500 (IL)

Kommentare:

Topoisomerase II is highly expressed in young seedlings. The protein is localized in the nucleus and gene expression levels are increased in proliferative tissues like shoot apex or root tip.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 200 μL of sterile water

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Referenzen für anti-TOP2 Antikörper (ABIN334538)

Xie, Lam: "Characterization of a DNA Topoisomerase II cDNA from Arabidopsis thaliana." in: Plant physiology, Vol. 106, Issue 4, pp. 1701-2, (1995) (PubMed).

Details zu TOP2

Target: TOP2 (DNA Topoisomerase II (TOP2))

Andere Bezeichnung: TOP2

Hintergrund: AGI Code: At3g23890
Topoisomerase type II (EC5.99.1.3) is one of the enzymes which is catalyzing unknotting of DNA by creating transient breaks in the DNA using a conserved tyrosine as the catalytic residue.Synonyme names of this protein: At3g23890, ATTOPII, DNA topoisomerase 2, DNA topoisomerase II, F14O13.7, TOP2, TOPOISOMERASE II

Molekulargewicht: expected: 164 kDa, apparent: 170 kDa (Arabidopsis thaliana)

UniProt: P30182

Pathways: Mitotic G1-G1/S Phases