TOP2 Antikörper (C-Term)

Produktnummer ABIN334538

Es gibt 1 Publikation für dieses Produkt. Der Kaninchen Polyklonal anti-TOP2 Antikörper wird verwendet zum Nachweis von TOP2 in Proben von Arabidopsis thaliana und Vicia faba. Er wurde validiert für WB und IL.

Kurzübersicht für TOP2 Antikörper (C-Term) (ABIN334538)

Target: TOP2 (DNA Topoisomerase II (TOP2))

Reaktivität: Arabidopsis thaliana, Vicia faba

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TOP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunolocalization (IL)

Produktdetails anti-TOP2 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Anti-TOP2 | DNA topoisomerase II

Spezifität: Antibody detects a protein of ca. 170 kDa on western blots of Arabidopsis thaliana protein extracts. In subcellular fractions of cultured Arabidopsis cells the antibody detects a 170 kDa protein exclusively in the nulear fraction.

Kreuzreaktivität (Details): Arabidopsis thaliana, Vicia faba
Not reactive in: Nicotiana tabacum

Homologie: Brassica rapa,Chlamydomonas reinhardtii,Chlorella vulgaris,Citrus clementina,Glycine max,Hordeum vulgare,Medicago truncatula,Oryza sativa,Ostreococcus tauri,Panicum italicum,Phaseolus vulgaris,Physcomitrium patens,Pinus sitcHensis,Populus trichocarpa,Solanum tuberosum,Sorghum bicolor,Triticum aestivum,Vitis vinifera,Volvox Caterii

Aufreinigung: Serum

Immunogen: The C-terminal 153 amino acids of the Arabidopsis thaliana Topoisomerase II (At3g23890

Anwendungsinformationen

Applikationshinweise: 1 : 500 (IL), 1 : 2000 (WB)

Kommentare:

Topoisomerase II is highly expressed in young seedlings. The protein is localized in the nucleus and gene expression levels are increased in proliferative tissues like shoot apex or root tip.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 200 μL of sterile water

Buffer: Serum

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Referenzen für anti-TOP2 Antikörper (ABIN334538)

Xie, Lam: "Characterization of a DNA Topoisomerase II cDNA from Arabidopsis thaliana." in: Plant physiology, Vol. 106, Issue 4, pp. 1701-2, (1995) (PubMed).

Details zu TOP2

Target: TOP2 (DNA Topoisomerase II (TOP2))

Andere Bezeichnung: TOP2

Hintergrund:

Background: Topoisomerase type II (EC5.99.1.3) is one of the enzymes which is catalyzing unknotting of DNA by creating transient breaks in the DNA using a conserved tyrosine as the catalytic residue.Synonyme names of this protein: At3g23890, ATTOPII, DNA topoisomerase 2, DNA topoisomerase II, F14O13.7, TOP2, TOPOISOMERASE II

Additional information: Antibody detects a protein of ca. 170 kDa on western blots of Arabidopsis thaliana protein extracts. In subcellular fractions of cultured Arabidopsis thaliana cells the antibody detects a 170 kDa protein exclusively in the nulear fraction (see picture).

Molekulargewicht: 164 | 170 kDa (Arabidopsis thaliana)

Gen-ID: 821972

UniProt: P30182

Pathways: Mitotic G1-G1/S Phases